human her2 positive breast cancer cell lines hcc 1954  (ATCC)

Journal: International Journal of Molecular Medicine

Article Title: Epigenetically altered miR-193a-3p promotes HER2 positive breast cancer aggressiveness by targeting GRB7

doi: 10.3892/ijmm.2019.4167

Figure Lengend Snippet: Expression of miR-193a-3p is decreased in HER2 positive breast cancer and is associated with tumor stage and grade. (A) RT-qPCR was conducted to test the expression of miR-193a-3p in 35 pairs of HER2 positive breast cancer tissues and adjacent tissues. (B) The expression of miR-193a-3p in HER2 positive breast cancer tissues at different stages (Stage 1, n=7; Stage 2, n=11; Stage 3, n=12; Stage 4, n=5). (C) The expression of miR-193a-3p in HER2 positive breast cancer tissues at different grades (Grade 1, n=14; Grade 2, n=12; Grade 3, n=9). (D) RT-qPCR was conducted to determine the expression of miR-193a-3p in normal human breast cells and HER2 positive breast cancer cells. ** P<0.01 and *** P<0.001, as indicated. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; HER2, human epidermal growth factor receptor 2; miR, microRNA.

Article Snippet: The human HER2 positive breast cancer cell lines HCC-1954, 21MT1 and JimT1 and human normal breast cell line MCF-10A were bought from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: International Journal of Molecular Medicine

Article Title: Epigenetically altered miR-193a-3p promotes HER2 positive breast cancer aggressiveness by targeting GRB7

doi: 10.3892/ijmm.2019.4167

Figure Lengend Snippet: Identification of DNA methylation leads to the downregulation of miR-193a-3p in breast cancer cells. (A) Quantitative polymerase chain reaction was carried out to determine the expression of miR-193a-3p in HER2 positive breast cancer cells following 4-days treatment with 5-Aza-dc. ** P<0.01 vs. DMSO. (B) Pyrosequencing analysis was conducted to analyze the percentage of methylation in the miR-193-3p promoter in HER2 positive breast cancer tissues at different stages (Stage 1, n=7; Stage 2, n=11; Stage 3, n=12; Stage 4, n=5). (C) Pyrosequencing analysis was conducted to analyze the percentage of methylation in the miR-193-3p promoter in HER2 positive breast cancer tissues at different grades (Grade 1, n=14; Grade 2, n=12; Grade 3, n=9). * P<0.05 and *** P<0.001, as indicated. HER2, human epidermal growth factor receptor 2; miR, microRNA.

Article Snippet: The human HER2 positive breast cancer cell lines HCC-1954, 21MT1 and JimT1 and human normal breast cell line MCF-10A were bought from the American Type Culture Collection (Manassas, VA, USA).

Techniques: DNA Methylation Assay, Real-time Polymerase Chain Reaction, Expressing, Methylation

Journal: International Journal of Molecular Medicine

Article Title: Epigenetically altered miR-193a-3p promotes HER2 positive breast cancer aggressiveness by targeting GRB7

doi: 10.3892/ijmm.2019.4167

Figure Lengend Snippet: Overexpression of miR-193a-3p inhibits proliferation, migration and invasion of HER2 positive breast cancer cells. (A) miR-193-3p mimics were transfected into 3 HER2 positive breast cancer cell lines and the expression of miR-193a-3p was significantly increased compared with the miR group. (B) Cell vitality was significantly downregulated by miR-193a-3p overexpression compared with the miR group. (C) The colony formation capacity was significantly repressed by miR-193a-3p overexpression compared with the miR group. (D) Cell invasion and migration abilities were significantly inhibited by miR-193a-3p overexpression compared with the miR group. * P<0.05 and ** P<0.01 vs. the miR group. OD, optical density; miR, microRNA; HER2, human epidermal growth factor receptor 2.

Article Snippet: The human HER2 positive breast cancer cell lines HCC-1954, 21MT1 and JimT1 and human normal breast cell line MCF-10A were bought from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Over Expression, Migration, Transfection, Expressing

Journal: International Journal of Molecular Medicine

Article Title: Epigenetically altered miR-193a-3p promotes HER2 positive breast cancer aggressiveness by targeting GRB7

doi: 10.3892/ijmm.2019.4167

Figure Lengend Snippet: miR-193a-3p directly inhibits the expression of GRB7 through targeting its 3′-UTR. (A) Western blotting and (B) statistical analysis of the expression of GRB7, which was significantly reduced by overexpression of miR-193a-3p in 3 HER2 positive breast cancer cell lines. miR-193a-3p could directly target 3′-UTR of GRB7 demonstrated by a (C) Cell Counting Kit-8 and (D) colony formation assay. ** P<0.01 vs. the miR group. (E) GRB7 was significantly upregu-lated in 35 pairs of HER2 positive breast cancer tissues. *** P<0.001, as indicated. (F) RNA immunoprecipitation assay demonstrating the association of GRB7 with miR-193a-3p in HCC-1954 cells. ** P<0.01 vs. the miR group. miR, microRNA; HER2, human epidermal growth factor receptor 2; UTR, untranslated region; GRB7, growth factor receptor bound protein 7; NC, negative control; OD, optical density.

Article Snippet: The human HER2 positive breast cancer cell lines HCC-1954, 21MT1 and JimT1 and human normal breast cell line MCF-10A were bought from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Western Blot, Over Expression, Cell Counting, Colony Assay, RNA Immunoprecipitation, Negative Control

Journal: International Journal of Molecular Medicine

Article Title: Epigenetically altered miR-193a-3p promotes HER2 positive breast cancer aggressiveness by targeting GRB7

doi: 10.3892/ijmm.2019.4167

Figure Lengend Snippet: GRB7 overexpression abolishes the inhibitory effect of miR-193a-3p on the oncogenic capacity of breast cancer. (A) Western blotting and (B) statistical analysis of the overexpression of GRB7 which abolished the decrease of GRB7 due to overexpression of miR-193a-3p in HER2 positive breast cancer cells. * P<0.05 and ** P<0.01, as indicated. (C) Cell vitality was significantly increased. ** P<0.01. (D) The colony formation capacity was significantly promoted. ** P<0.01, as indicated. (E) Cell invasion and (F) migration abilities were significantly accelerated by GRB7 overexpression. * P<0.05 and ** P<0.01. GRB7, growth factor receptor bound protein 7; NC, negative control; OD, optical density; miR, microRNA.

Article Snippet: The human HER2 positive breast cancer cell lines HCC-1954, 21MT1 and JimT1 and human normal breast cell line MCF-10A were bought from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Over Expression, Western Blot, Migration, Negative Control

Journal: International Journal of Molecular Medicine

Article Title: Epigenetically altered miR-193a-3p promotes HER2 positive breast cancer aggressiveness by targeting GRB7

doi: 10.3892/ijmm.2019.4167

Figure Lengend Snippet: miR-193a-3p may inhibit HER2 positive breast cancer through downregulating GRB7 and inactivating the ERK/FOXM1 signaling pathway. (A) Western blotting and (B) statistical analysis of the protein level of pERK 1/2 and (C) FOXM1 were significantly decreased by GRB7 overexpression in HCC-1954 cells overexpressing miR-193a-3p. * P<0.05 and ** P<0.01, as indicated. miR, microRNA; pERK, phosphorylated extracellular signal regulated kinase; FOXM1, forkhead box M1; HER2, human epidermal growth factor receptor 2; GRB7, growth factor receptor bound protein 7.

Article Snippet: The human HER2 positive breast cancer cell lines HCC-1954, 21MT1 and JimT1 and human normal breast cell line MCF-10A were bought from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Western Blot, Over Expression